2019-02-19 19:42:332018-09-21 09:48:19
geneLength
858
855
Gene
Coordinates
3,808,512 3,809,369
3,808,512 → 3,809,369
The protein
[SW|Domains]
2 x Dihydroxyacetone phosphate binding domain (210212), (231234)
2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
Biological materials
Mutant
GP591 (''fbaA''::''cat''), available in [SW|Jrg Stlke]'s lab, [Pubmed|23420519]
GP596 (''fbaA''::''erm''), available in [SW|Jrg Stlke]'s lab, [Pubmed|23420519]
BKE37120 ([[gene|fbaA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
BKK37120 ([[gene|fbaA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
GP591 (''fbaA''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP596 (''fbaA''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
BKE37120 (Δ[[gene|fbaA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
BKK37120 (Δ[[gene|fbaA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
Biological materials
Expression vectors
for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1423, available in [SW|Jrg Stlke]'s lab
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP88, available in [SW|Jrg Stlke]'s lab, [pubmed|19193632]
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP395, available in [SW|Jrg Stlke]'s lab
Biological materials
lacZ fusion
pGP601 (in [[protein|pAC6]]) [pubmed|11489127]
pGP601 (in [[protein|pAC6]])
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab, [pubmed|19193632]
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
Biological materials
Expression vector
for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1423, available in [SW|Jörg Stülke]'s lab
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP88, available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP395, available in [SW|Jörg Stülke]'s lab